Journal: Advanced photonics

Article Title: Resolution enhancement with deblurring by pixel reassignment

doi: 10.1117/1.ap.5.6.066004

Figure Lengend Snippet: Comparison of DPR, SRRF, and MSSR with optical pixel reassignment and deconvolution. (a) Left, BPAE cells imaged using optical pixel reassignment and deconvolution with Nikon confocal microscope without (top) and with SoRa superresolution enhanced by RL deconvolution (20 iterations). Right, confocal images deblurred by RL deconvolution (20 iterations), DPR (gains 1 and 2), SRRF, and MSSR. DPR parameters: PSF FWHM, 2 pixels; local-minimum filter radius, 40 pixels. MSSR parameters: PSF FWHM, 2 pixels; magnification, 4; order, 1. SRRF: ring radius, 0.5; magnification, 4; axes, 6. Scale bar, 2 μ m. (b) Zoom-in ROIs indicated by red squares in (a). Scale bar, 600 nm.

Article Snippet: To serve as a surrogate ground truth, we obtained images of BPAE cells with a state-of-the-art Nikon CS-WU1 spinning disk microscope equipped with a superresolution module (SoRa) based on optical pixel reassignment (objective, 60× oil, 1.42 NA; PSF FWHM, 162.5 nm—conventional configuration, 114.9 nm—SoRa configuration; pixel size, 108.3 nm—conventional configuration, 27.1 nm—SoRa configuration), to which we additionally applied 20 iterations of RL deconvolution using the software supplied by the manufacturer.

Techniques: Comparison, Microscopy